Keratin 80 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-16952R-Cy5.5 |
| Product Name | Keratin 80 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Keratin 80 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 144501 |
| Swiss Prot | Q6KB66 |
| Subcellular location | Cytoplasm |
| Synonyms | CK 80; CK-80; CK80; Cytokeratin 80; Cytokeratin-80; K 80; K2C80_HUMAN; K80; KB20; Keratin 80; Keratin; Keratin-80; KRT 80; Krt80; type II cytoskeletal 80; Type II keratin; Type-II keratin Kb20. |
| Background | There are two types of cytoskeletal and microfibrillar keratin, I (acidic) and II (neutral to basic) (40-55 and 56-70 kDa, respectively). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Keratin 80 Polyclonal Antibody, Cy5.5 Conjugated (BS-16952R-CY5-5) validated for, and at what dilution?
BS-16952R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Keratin 80 Polyclonal Antibody, Cy5.5 Conjugated (BS-16952R-CY5-5) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog.
How should BS-16952R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Keratin 80 Polyclonal Antibody, Cy5.5 Conjugated (BS-16952R-CY5-5)?
KLH conjugated synthetic peptide derived from human Keratin 80
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-16952R-CY5-5)?
Cy5.5 on BS-16952R-CY5-5: excitation / emission 675nm/694nm.