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ox-LDL Polyclonal Antibody, PE-Cy5 Conjugated

Applications

  • FCM

Reactivity

  • Human
Overview
Catalog # bs-1698R-PE-Cy5-100uL
Product Name ox-LDL Polyclonal Antibody, PE-Cy5 Conjugated
Applications
FCM
Reactivity Human
Specifications
Conjugation PE-Cy5
Host Rabbit
Source Full length plasma protein (Human)
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Secreted, Cell membrane
Synonyms ox-LDL: Cu2SO4 oxidized low density lipoprotein; oxidized low density lipoprotein; Low density lipoprotein; MDA oxidized LDL; MDA oxidized low density lipoprotein.
Background Low-density lipoprotein (LDL) is the carrier protein for cholesterol in the blood. LDL binds to its receptor on the capillary walls and thereby mediates the uptake and clearence of cholesterol from the circulation. In atherosclerotic lesions oxidatively modified LDL is found and oxidized LDL is specifically recognized and ingested by macrophages via scavenger receptor A and CD36. Oxidized LDL may be a marker of atherosclerosis but the precise changes in oxidized LDL are not well described. Low-density lipoprotein oxidised with Cu2SO4.
Application Dilution
FCM 1:20-100

Frequently asked questions

Which applications is ox-LDL Polyclonal Antibody, PE-Cy5 Conjugated (bs-1698R-PE-Cy5) validated for, and at what dilution?

bs-1698R-PE-Cy5 is validated for FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ox-LDL Polyclonal Antibody, PE-Cy5 Conjugated (bs-1698R-PE-Cy5) react with?

Tested reactivity: Human.

How should bs-1698R-PE-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ox-LDL Polyclonal Antibody, PE-Cy5 Conjugated (bs-1698R-PE-Cy5)?

Full length plasma protein (Human)

What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-1698R-PE-Cy5)?

PE-Cy5 on bs-1698R-PE-Cy5: excitation / emission 488nm/670nm.

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