ox-LDL Polyclonal Antibody, PerCP Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-1698R-PerCP-100uL |
| Product Name | ox-LDL Polyclonal Antibody, PerCP Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | Full length plasma protein (Human) |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Secreted, Cell membrane |
| Synonyms | ox-LDL: Cu2SO4 oxidized low density lipoprotein; oxidized low density lipoprotein; Low density lipoprotein; MDA oxidized LDL; MDA oxidized low density lipoprotein. |
| Background | Low-density lipoprotein (LDL) is the carrier protein for cholesterol in the blood. LDL binds to its receptor on the capillary walls and thereby mediates the uptake and clearence of cholesterol from the circulation. In atherosclerotic lesions oxidatively modified LDL is found and oxidized LDL is specifically recognized and ingested by macrophages via scavenger receptor A and CD36. Oxidized LDL may be a marker of atherosclerosis but the precise changes in oxidized LDL are not well described. Low-density lipoprotein oxidised with Cu2SO4. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ox-LDL Polyclonal Antibody, PerCP Conjugated (bs-1698R-PerCP) validated for, and at what dilution?
bs-1698R-PerCP is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ox-LDL Polyclonal Antibody, PerCP Conjugated (bs-1698R-PerCP) react with?
Tested reactivity: Human.
How should bs-1698R-PerCP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ox-LDL Polyclonal Antibody, PerCP Conjugated (bs-1698R-PerCP)?
Full length plasma protein (Human)
What are the excitation and emission wavelengths of the PerCP conjugate (bs-1698R-PerCP)?
PerCP on bs-1698R-PerCP: excitation / emission 482nm/677nm.