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Galectin 9 Polyclonal Antibody, APC Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-F)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Cow
  • Pig
Overview
Catalog # bs-1699R-APC
Product Name Galectin 9 Polyclonal Antibody, APC Conjugated
Applications
WB FCM IF(IHC-F)
Reactivity Human, Mouse
Predicted Reactivity Rat, Cow, Pig
Specifications
Conjugation APC
Host Rabbit
Source KLH conjugated synthetic peptide derived from mouse Galectin
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3965
Subcellular location Cytoplasm, Secreted
Synonyms Ecalectin; galectin9; HOM HD 21; HOMHD21; HUAT; Lectin galactoside binding soluble 9; LGAL S9; LGALS 9; LGALS9; LGALS9A; MGC117375; MGC125973; MGC125974 ; Urate transporter/channel protein; Galectin-9; Gal-9.
Background The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin 9 is an S-type lectin and an eosinophil chemoattractant (ECA) produced by antigen-activated T cells. Galectin 9 is involved in apoptosis, is thought to be a prognostic factor for breast cancer and is also overexpressed in Hodgkin's disease tissue. The protein has N- and C- terminal carbohydrate-binding domains connected by a link peptide. Multiple alternatively spliced transcript variants have been found for this gene.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-F) 1:50-200

Frequently asked questions

Which applications is Galectin 9 Polyclonal Antibody, APC Conjugated (bs-1699R-APC) validated for, and at what dilution?

bs-1699R-APC is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-F) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Galectin 9 Polyclonal Antibody, APC Conjugated (bs-1699R-APC) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Cow, Pig.

How should bs-1699R-APC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Galectin 9 Polyclonal Antibody, APC Conjugated (bs-1699R-APC)?

KLH conjugated synthetic peptide derived from mouse Galectin

What are the excitation and emission wavelengths of the APC conjugate (bs-1699R-APC)?

APC on bs-1699R-APC: excitation / emission 650nm/660nm.