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KIF2A Polyclonal Antibody, Cy3 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
Overview
Catalog # bs-17055R-Cy3
Product Name KIF2A Polyclonal Antibody, Cy3 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Cow, Sheep, Pig, Horse, Chicken
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human KIF2A
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3796
Swiss Prot O00139
Subcellular location Cytoplasm
Synonyms HK2; kif2; Kif2a; KIF2A_HUMAN; Kinesin 2; Kinesin heavy chain 2; Kinesin heavy chain member 2; Kinesin heavy chain member 2A; Kinesin like protein KIF2A; Kinesin-2; Kinesin-like protein KIF2A; kns2; M Kinesin; M-Kinesin.
Background Plus end-directed microtubule-dependent motor required for normal brain development. May regulate microtubule dynamics during axonal growth. Required for normal progression through mitosis. Required for normal congress of chromosomes at the metaphase plate. Required for normal spindle dynamics during mitosis. Promotes spindle turnover. Implicated in formation of bipolar mitotic spindles. Has microtubule depolymerization activity.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is KIF2A Polyclonal Antibody, Cy3 Conjugated (bs-17055R-Cy3) validated for, and at what dilution?

bs-17055R-Cy3 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does KIF2A Polyclonal Antibody, Cy3 Conjugated (bs-17055R-Cy3) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Pig, Horse, Chicken.

How should bs-17055R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise KIF2A Polyclonal Antibody, Cy3 Conjugated (bs-17055R-Cy3)?

KLH conjugated synthetic peptide derived from human KIF2A

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-17055R-Cy3)?

Cy3 on bs-17055R-Cy3: excitation / emission 512,550nm/570,615nm.