Angiotensin I Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1707R-APC-Cy7-100uL |
| Product Name | Angiotensin I Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | Immunogen used for this antibody is DRVYIHPFHL, corresponding to amino acids 34-43 of human Angiotensinogen. |
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Sheep, Horse |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide of human Angiotensin I |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 183 |
| Subcellular location | Secreted |
| Synonyms | AGT; Alpha 1 antiproteinase antitrypsin; Ang I; Ang II; Ang III; Angiotensin 1; Angiotensin; Angiotensin I; Angiotensin II; Angiotensin-2; Angiotensin III; Angiotensin-3; Angiotensinogen; Angiotensinogen serpin peptidase inhibitor clade A member 8; ANGT_HUMAN; Angiotensin 1-10; Angiotensin 1-8; ANHU; Des-Asp[1]-angiotensin II; FLJ92595; FLJ97926; OTTHUMP00000035878; Pre angiotensinogen; Serine or cysteine proteinase inhibitor; Serpin A8; Serpin peptidase inhibitor, clade A, member 8; SERPINA8. |
| Background | Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. Angiotensin-2: acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone. Angiotensin-3: stimulates aldosterone release. Angiotensin 1-7: is a ligand for the G-protein coupled receptor MAS1. Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Angiotensin I Polyclonal Antibody, APC-Cy7 Conjugated (bs-1707R-APC-Cy7) validated for, and at what dilution?
bs-1707R-APC-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Angiotensin I Polyclonal Antibody, APC-Cy7 Conjugated (bs-1707R-APC-Cy7) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Sheep, Horse.
How should bs-1707R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Angiotensin I Polyclonal Antibody, APC-Cy7 Conjugated (bs-1707R-APC-Cy7)?
KLH conjugated synthetic peptide of human Angiotensin I
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-1707R-APC-Cy7)?
APC-Cy7 on bs-1707R-APC-Cy7: excitation / emission 650nm/780nm.