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TRIM62 Polyclonal Antibody, AbBy Fluor-555 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Sheep
  • Rabbit
Overview
Catalog # bs-17118R-BF555-100uL
Product Name TRIM62 Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Sheep, Rabbit
Specifications
Conjugation AbBy Fluor-555
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TRIM62
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 55223
Swiss Prot Q9BVG3
Subcellular location Cytoplasm
Synonyms DEAR1; E3 ubiquitin-protein ligase TRIM62; Tripartite motif-containing protein 62; TRIM62
Background E3 ubiquitin ligase whose activity is dependent on E2 ubiquitin-conjugating enzyme UBE2D2.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is TRIM62 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-17118R-BF555) validated for, and at what dilution?

bs-17118R-BF555 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TRIM62 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-17118R-BF555) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Rabbit.

How should bs-17118R-BF555 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TRIM62 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-17118R-BF555)?

KLH conjugated synthetic peptide derived from human TRIM62

What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-17118R-BF555)?

AbBy Fluor-555 on bs-17118R-BF555: excitation / emission 553nm/568nm.