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TRIM68 Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
  • Cow
  • Sheep
  • Pig
  • Horse
Overview
Catalog # bs-17123R-Cy3-100uL
Product Name TRIM68 Polyclonal Antibody, Cy3 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Rat
Predicted Reactivity Mouse, Cow, Sheep, Pig, Horse
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TRIM68
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 55128
Swiss Prot Q6AZZ1
Subcellular location Cytoplasm
Synonyms E3 ubiquitin-protein ligase TRIM68; TRIM68; RING finger protein 137; RING-type E3 ubiquitin transferase TRIM68; SSA protein SS-56; SS-56; Tripartite motif-containing protein 68; GC109; RNF137; SS56
Background Functions as an ubiquitin E3 ligase. Acts as a coactivator of androgen receptor (AR) depending on its ubiquitin ligase activity.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is TRIM68 Polyclonal Antibody, Cy3 Conjugated (bs-17123R-Cy3) validated for, and at what dilution?

bs-17123R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TRIM68 Polyclonal Antibody, Cy3 Conjugated (bs-17123R-Cy3) react with?

Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Cow, Sheep, Pig, Horse.

How should bs-17123R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TRIM68 Polyclonal Antibody, Cy3 Conjugated (bs-17123R-Cy3)?

KLH conjugated synthetic peptide derived from human TRIM68

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-17123R-Cy3)?

Cy3 on bs-17123R-Cy3: excitation / emission 512,550nm/570,615nm.

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