phospho-SP1 (Thr739) Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-17137R-BF647-100uL |
| Product Name | phospho-SP1 (Thr739) Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF(ICC)
IF
|
| Reactivity | Human |
| Predicted Reactivity | Cow, Horse, Monkey |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthesised phosphopeptide derived from human SP1 around the phosphorylation site of Thr739 |
| Modification Site | Thr739 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6667 |
| Swiss Prot | P08047 |
| Synonyms | SP1 (phospho T739); SP1 (phospho-Thr739); SP1 (phospho-T739); p-SP1 (phospho T453); p-TSFP1 (phospho T453); Sp1 transcription factor isoform a; TSFP1; TSFP 1; Specificity protein 1; Transcription factor Sp1; SP 1; SP1; Sp1 transcription factor; SP1_HUMAN. |
| Background | Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| IF | |
Frequently asked questions
Which applications is phospho-SP1 (Thr739) Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-17137R-BF647) validated for, and at what dilution?
bs-17137R-BF647 is validated for WB (1:300-5000); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-SP1 (Thr739) Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-17137R-BF647) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Horse, Monkey.
How should bs-17137R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-SP1 (Thr739) Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-17137R-BF647)?
KLH conjugated synthesised phosphopeptide derived from human SP1 around the phosphorylation site of Thr739
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-17137R-BF647)?
AbBy Fluor-647 on bs-17137R-BF647: excitation / emission 650nm/665nm.