phospho-TPH2 (Ser19) Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-17166R-BF555 |
| Product Name | phospho-TPH2 (Ser19) Polyclonal Antibody, AbBy Fluor-555 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Dog, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthesised phosphopeptide derived from human TPH2 around the phosphorylation site of Ser19 |
| Modification Site | Ser19 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 121278 |
| Swiss Prot | Q8IWU9 |
| Subcellular location | Cytoplasm |
| Synonyms | NTPH; ADHD7; Tryptophan 5-hydroxylase 2; Neuronal tryptophan hydroxylase; Tryptophan 5-monooxygenase 2; TPH2 |
| Background | This gene encodes a member of the pterin-dependent aromatic acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. The human genome contains two related tryptophan hydroxylases, one on chromosome 11p15-p14 and one on chromosome 12q21. This gene is expressed predominantly in the brain stem. Mutations in this gene may be associated with psychiatric diseases such as bipolar affective disorder and major depression. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-TPH2 (Ser19) Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-17166R-BF555) validated for, and at what dilution?
bs-17166R-BF555 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-TPH2 (Ser19) Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-17166R-BF555) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Horse.
How should bs-17166R-BF555 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-TPH2 (Ser19) Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-17166R-BF555)?
KLH conjugated synthesised phosphopeptide derived from human TPH2 around the phosphorylation site of Ser19
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-17166R-BF555)?
AbBy Fluor-555 on bs-17166R-BF555: excitation / emission 553nm/568nm.