Cytokeratin 13 Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1717R-BF488 |
| Product Name | Cytokeratin 13 Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Sheep, Horse, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Cytokeratin-13 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3860 |
| Subcellular location | Cytoplasm |
| Synonyms | type I cytoskeletal 13; 47 kDa cytokeratin; CK-13; CK13; Cytokeratin 13; Cytokeratin-13; K13; K1C13_HUMAN; Ka13; Keratin 13; Keratin; keratin type I cytoskeletal 13; Keratin-13; Krt-1.13; Krt1-13; KRT13; MGC161462; MGC3781; Type I keratin Ka13; Keratin, type I cytoskeletal 13; K13. |
| Background | The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been described. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is Cytokeratin 13 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-1717R-BF488) validated for, and at what dilution?
bs-1717R-BF488 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Cytokeratin 13 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-1717R-BF488) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Sheep, Horse, Rabbit.
How should bs-1717R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Cytokeratin 13 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-1717R-BF488)?
KLH conjugated synthetic peptide derived from human Cytokeratin-13
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-1717R-BF488)?
AbBy Fluor-488 on bs-1717R-BF488: excitation / emission 499nm/519nm.