IVD Polyclonal Antibody

$line1,MCF-7 lysates\uff1bline2,mouse heart lysate probed with Rabbit Anti-IVD Polyclonal Antibody, Unconjugated (bs-17189R) at 1:300 in 4\u02daC overnight. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90min at RT.$

line1,MCF-7 lysates\uff1bline2,mouse heart lysate probed with Rabbit Anti-IVD Polyclonal Antibody, Unconjugated (bs-17189R) at 1:300 in 4\u02daC overnight. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90min at RT.

$Mouse kidney lysates probed with IVD Polyclonal Antibody, Unconjugated (bs-17189R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Mouse kidney lysates probed with IVD Polyclonal Antibody, Unconjugated (bs-17189R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

$25 ug total protein per lane of various lysates (see on figure) probed with IVD polyclonal antibody, unconjugated (bs-17189R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with IVD polyclonal antibody, unconjugated (bs-17189R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse

Predicted Reactivity

Human
Rat
Dog
Cow
Sheep
Pig
Horse
Rabbit

Overview
Catalog #	bs-17189R
Product Name	IVD Polyclonal Antibody
Applications	WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity	Mouse
Predicted Reactivity	Human, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human IVD
Immunogen Range	201-300/423
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3712
Swiss Prot	P26440
Subcellular location	Cytoplasm
Synonyms	ACAD2; Isovaleryl-CoA dehydrogenase, mitochondrial; IVD
Background	Isovaleryl-CoA dehydrogenase (IVD) is a mitochondrial matrix enzyme that catalyzes the third step in leucine catabolism. The genetic deficiency of IVD results in an accumulation of isovaleric acid, which is toxic to the central nervous system and leads to isovaleric acidemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009]
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200