SC65 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-17258R-Cy3-100uL |
| Product Name | SC65 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Pig, Macaque Monkey |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SC65 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10609 |
| Swiss Prot | Q92791 |
| Subcellular location | Nucleus |
| Synonyms | Leprecan-like protein 4; Leprel4; NOL55; Nucleolar autoantigen 55kDa; Nucleolar autoantigen No55; SC 65; SC65_HUMAN; Synaptonemal complex protein SC65. |
| Background | Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linking of collagen fibrils. Required for normal bone density and normal skin stability via its role in hydroxylation of lysine residues in collagen alpha chains and in collagen fibril assembly. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is SC65 Polyclonal Antibody, Cy3 Conjugated (bs-17258R-Cy3) validated for, and at what dilution?
bs-17258R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SC65 Polyclonal Antibody, Cy3 Conjugated (bs-17258R-Cy3) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Pig, Macaque Monkey.
How should bs-17258R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SC65 Polyclonal Antibody, Cy3 Conjugated (bs-17258R-Cy3)?
KLH conjugated synthetic peptide derived from human SC65
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-17258R-Cy3)?
Cy3 on bs-17258R-Cy3: excitation / emission 512,550nm/570,615nm.