Topoisomerase II beta Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-17324R-Biotin-100uL |
| Product Name | Topoisomerase II beta Polyclonal Antibody, Biotin Conjugated |
| Applications |
ELISA
IHC-P
IHC-F
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Cow, Sheep, Pig, Horse, Rabbit, Chimpanzee |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Topoisomerase II beta |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 7155 |
| Swiss Prot | Q02880 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Antigen MLAA 44; beta isozyme; DNA topoisomerase 2 beta; DNA topoisomerase 2-beta; DNA topoisomerase II 180 kD; DNA topoisomerase II; DNA topoisomerase II beta; DNA topoisomerase II beta isozyme; Top 2; TOP 2B; TOP IIB; Top2 beta; TOP2B; TOP2B_HUMAN; Top2beta; TOPIIB; Topo II beta; Topoisomerase (DNA) II beta 180kDa; Topoisomerase (DNA) II beta; Topoisomerase IIb; U937 associated antigen. |
| Background | Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Indirectly ivolved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is Topoisomerase II beta Polyclonal Antibody, Biotin Conjugated (bs-17324R-Biotin) validated for, and at what dilution?
bs-17324R-Biotin is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Topoisomerase II beta Polyclonal Antibody, Biotin Conjugated (bs-17324R-Biotin) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Sheep, Pig, Horse, Rabbit, Chimpanzee.
How should bs-17324R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Topoisomerase II beta Polyclonal Antibody, Biotin Conjugated (bs-17324R-Biotin)?
KLH conjugated synthetic peptide derived from human Topoisomerase II beta