phospho C-Myc (Thr58 / Ser62) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1732R-Cy3 |
| Product Name | phospho C-Myc (Thr58 / Ser62) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human c-Myc around the phosphorylation site of Thr58+Ser62 |
| Modification Site | Thr58+Ser62 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4609 |
| Swiss Prot | P01106 |
| Subcellular location | Nucleus |
| Synonyms | MRTL; MYCC; c-Myc; bHLHe39; Myc proto-oncogene protein; Class E basic helix-loop-helix protein 39; Proto-oncogene c-Myc; Transcription factor p64; MYC |
| Background | Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
Frequently asked questions
Which applications is phospho C-Myc (Thr58 / Ser62) Polyclonal Antibody, Cy3 Conjugated (bs-1732R-Cy3) validated for, and at what dilution?
bs-1732R-Cy3 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho C-Myc (Thr58 / Ser62) Polyclonal Antibody, Cy3 Conjugated (bs-1732R-Cy3) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse.
How should bs-1732R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho C-Myc (Thr58 / Ser62) Polyclonal Antibody, Cy3 Conjugated (bs-1732R-Cy3)?
KLH conjugated synthetic phosphopeptide derived from human c-Myc around the phosphorylation site of Thr58+Ser62
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-1732R-Cy3)?
Cy3 on bs-1732R-Cy3: excitation / emission 512,550nm/570,615nm.