phospho-c-Jun (Thr91+Thr93) Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1735R-RBITC |
| Product Name | phospho-c-Jun (Thr91+Thr93) Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep, Pig, Chicken, Rabbit |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human c-Jun around the phosphorylation site of Thr91/93 |
| Modification Site | Thr91+Thr93 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3725 |
| Subcellular location | Nucleus |
| Synonyms | p-c-JunThr91+Thr93; p-JunThr91+Thr93; Jun oncogene; JUN; Activator Protein 1; AP 1; AP1; AP-1; Enhancer Binding Protein AP1; Jun Activation Domain Binding Protein; JUN protein; JUNC; p39; Proto oncogene cJun; Transcription Factor AP1; V jun avian sarcoma virus 17 oncogene homolog; vJun Avian Sarcoma Virus 17 Oncogene Homolog; JUN_HUMAN; Transcription factor AP-1; Activator 1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog. |
| Background | C-jun (Oncoprotein C-jun) is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements and appears to be a major downstream target of the SAPK/JNK signaling pathway. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress induced signaling pathways. The MAP kinase homologue, SAPK/JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-c-Jun (Thr91+Thr93) Polyclonal Antibody, RBITC Conjugated (bs-1735R-RBITC) validated for, and at what dilution?
bs-1735R-RBITC is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-c-Jun (Thr91+Thr93) Polyclonal Antibody, RBITC Conjugated (bs-1735R-RBITC) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Chicken, Rabbit.
How should bs-1735R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-c-Jun (Thr91+Thr93) Polyclonal Antibody, RBITC Conjugated (bs-1735R-RBITC)?
KLH conjugated synthetic phosphopeptide derived from human c-Jun around the phosphorylation site of Thr91/93
What are the excitation and emission wavelengths of the RBITC conjugate (bs-1735R-RBITC)?
RBITC on bs-1735R-RBITC: excitation / emission 540nm/625nm.