HS2ST1 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-17389R-RBITC |
| Product Name | HS2ST1 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, Zebrafish |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HS2ST1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9653 |
| Swiss Prot | Q7LGA3 |
| Subcellular location | Cytoplasm |
| Synonyms | 2-O-sulfotransferase; 2OST; dJ604K5.2; FLJ11317; heparan sulfate 2 O sulfotransferase 1; Heparan sulfate 2-O-sulfotransferase 1; HS2ST_HUMAN; hs2st1; KIAA0448; MGC131986. |
| Background | Heparan sulfate structures, which are responsible for executing multiple biologic activities, are generated and regulated by heparan sulfate biosynthetic enzymes. HS2ST1 is a 356 amino acid single-pass type II membrane protein that localizes to the golgi apparatus and belongs to the sulfotransferase 3 family. Expressed as multiple alternatively spliced isoforms, HS2ST1 functions to catalyze the transfer of sulfate groups to hexuronic acid residues within maturing heparan sulfate (HS), an event which is crucial for proper HS-related ligand binding and signaling processes. HS2ST1 is subject to post-translational N-glycosylation and, in addition to its role in HS function, may be involved in proper kidney formation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is HS2ST1 Polyclonal Antibody, RBITC Conjugated (bs-17389R-RBITC) validated for, and at what dilution?
bs-17389R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HS2ST1 Polyclonal Antibody, RBITC Conjugated (bs-17389R-RBITC) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, Zebrafish.
How should bs-17389R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HS2ST1 Polyclonal Antibody, RBITC Conjugated (bs-17389R-RBITC)?
KLH conjugated synthetic peptide derived from human HS2ST1
What are the excitation and emission wavelengths of the RBITC conjugate (bs-17389R-RBITC)?
RBITC on bs-17389R-RBITC: excitation / emission 540nm/625nm.