HIPK1 Polyclonal Antibody, PerCP-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-17403r-percp-cy7-100uL |
| Product Name | HIPK1 Polyclonal Antibody, PerCP-Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Monkey |
| Predicted Reactivity | Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit, Monkey |
| Specifications | |
| Conjugation | PerCP-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HIPK1 |
| Immunogen Range | 1-100/1210 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 204851 |
| Swiss Prot | Q86Z02 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Hipk 1; Hipk1; HIPK1_HUMAN; Homeodomain interacting protein kinase 1; Homeodomain interacting protein kinase 1 like protein; Homeodomain-interacting protein kinase 1; Myak; Nbak 2; Nbak2; Nuclear body associated kinase 2; Nuclear body associated kinase 2b |
| Background | Serine/threonine-protein kinase involved in transcription regulation and TNF-mediated cellular apoptosis. Plays a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX and MYB. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. Inactivates MYB transcription factor activity by phosphorylation. Prevents MAP3K5-JNK activation in the absence of TNF. TNF triggers its translocation to the cytoplasm in response to stress stimuli, thus activating nuclear MAP3K5-JNK by derepression and promoting apoptosis. May be involved in anti-oxidative stress responses. Involved in the regulation of eye size, lens formation and retinal lamination during late embryogenesis. Promotes angiogenesis and to be involved in erythroid differentiation. May be involved in malignant squamous cell tumor formation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is HIPK1 Polyclonal Antibody, PerCP-Cy7 Conjugated (bs-17403R-PerCP-Cy7) validated for, and at what dilution?
bs-17403R-PerCP-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HIPK1 Polyclonal Antibody, PerCP-Cy7 Conjugated (bs-17403R-PerCP-Cy7) react with?
Tested reactivity: Human, Mouse, Monkey. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit, Monkey.
How should bs-17403R-PerCP-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HIPK1 Polyclonal Antibody, PerCP-Cy7 Conjugated (bs-17403R-PerCP-Cy7)?
KLH conjugated synthetic peptide derived from human HIPK1 Immunogen range: 1-100/1210.
What are the excitation and emission wavelengths of the PerCP-Cy7 conjugate (bs-17403R-PerCP-Cy7)?
PerCP-Cy7 on bs-17403R-PerCP-Cy7: excitation / emission 482nm/780nm.