alpha Actinin 4 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-1741R-RBITC |
| Product Name | alpha Actinin 4 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Actinin alpha 4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 81 |
| Synonyms | Actinin alpha 4; alpha-Actinin 4; alpha Actinin-4; actinin 4; actinin4; actinin-4; ACTN 4; ACTN4; DKFZp686K23158; F actin cross linking protein; FSGS 1; FSGS; FSGS1; Non muscle alpha actinin 4. |
| Background | Loading Control Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF | FCM1:100 |
Frequently asked questions
Which applications is alpha Actinin 4 Polyclonal Antibody, RBITC Conjugated (bs-1741R-RBITC) validated for, and at what dilution?
bs-1741R-RBITC is validated for WB (1:500-2000); FCM (1:100); IF(ICC) (1:100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does alpha Actinin 4 Polyclonal Antibody, RBITC Conjugated (bs-1741R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-1741R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise alpha Actinin 4 Polyclonal Antibody, RBITC Conjugated (bs-1741R-RBITC)?
KLH conjugated synthetic peptide derived from human Actinin alpha 4
What are the excitation and emission wavelengths of the RBITC conjugate (bs-1741R-RBITC)?
RBITC on bs-1741R-RBITC: excitation / emission 540nm/625nm.