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Histone H1.X Polyclonal Antibody, PE Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
Overview
Catalog # bs-17424R-PE
Product Name Histone H1.X Polyclonal Antibody, PE Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse
Predicted Reactivity Rat, Dog, Cow, Sheep, Pig
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Histone H1.X
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 8971
Swiss Prot Q92522
Subcellular location Nucleus
Synonyms H1 histone family member X; Histone H1fx; H1FX; H1X_HUMAN_ H1X; Histone H1x; MGC15959; MGC8350.
Background Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H1 family. [provided by RefSeq, Jul 2008]
Application Dilution
WB 1:300-5000
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Frequently asked questions

Which applications is Histone H1.X Polyclonal Antibody, PE Conjugated (bs-17424R-PE) validated for, and at what dilution?

bs-17424R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Histone H1.X Polyclonal Antibody, PE Conjugated (bs-17424R-PE) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep, Pig.

How should bs-17424R-PE be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Histone H1.X Polyclonal Antibody, PE Conjugated (bs-17424R-PE)?

KLH conjugated synthetic peptide derived from human Histone H1.X

What are the excitation and emission wavelengths of the PE conjugate (bs-17424R-PE)?

PE on bs-17424R-PE: excitation / emission 496,564nm/578nm.