p21-ARC Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-17589R-BF680 |
| Product Name | p21-ARC Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Cow, Chicken, Rabbit, Xenopuslaevis |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human p21-ARC |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10094 |
| Swiss Prot | O15145 |
| Subcellular location | Cytoplasm |
| Synonyms | ARC21; p21-Arc; Actin-related protein 2/3 complex subunit 3; Arp2/3 complex 21 kDa subunit; ARPC3 |
| Background | Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is p21-ARC Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-17589R-BF680) validated for, and at what dilution?
bs-17589R-BF680 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does p21-ARC Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-17589R-BF680) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Cow, Chicken, Rabbit, Xenopuslaevis.
How should bs-17589R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise p21-ARC Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-17589R-BF680)?
KLH conjugated synthetic peptide derived from human p21-ARC
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-17589R-BF680)?
AbBy Fluor-680 on bs-17589R-BF680: excitation / emission 679nm/702nm.