SPATA5L1 Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-17632R-BF680 |
| Product Name | SPATA5L1 Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SPATA5L1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 79029 |
| Swiss Prot | Q9BVQ7 |
| Subcellular location | Cytoplasm |
| Synonyms | FLJ12286; MGC5347; SPA5L_HUMAN; SPATA5L1; Spermatogenesis-associated protein 5-like protein 1. |
| Background | SPATA5L1 is a 753 amino acid protein belonging to the AAA ATPase family and AFG2 subfamily. Single nucleotide polymorphisms (SNPs) present in SPATA5L1 at the glycine amidinotransferase (GATM)-SPATA5L1 locus have been found to correlate with glomerular filtration rate (GFR), having significant implications for kidney disease research. SPATA5L1 localizes to cytoplasm and exists as three alternatively spliced isoforms. The gene encoding SPATA5L1 maps to human chromosome 15q21.1. Encoding more than 700 genes, chromosome 15 is made up of approximately 106 million base pairs and comprises about 3% of the human genome. Tay-Sachs disease is a lethal disorder associated with mutations of the HEXA gene, which is encoded by chromosome 15. Marfan syndrome is associated with chromosome 15 through the FBN1 gene. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is SPATA5L1 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-17632R-BF680) validated for, and at what dilution?
bs-17632R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SPATA5L1 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-17632R-BF680) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Rabbit.
How should bs-17632R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SPATA5L1 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-17632R-BF680)?
KLH conjugated synthetic peptide derived from human SPATA5L1
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-17632R-BF680)?
AbBy Fluor-680 on bs-17632R-BF680: excitation / emission 679nm/702nm.