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SPIB Polyclonal Antibody, Cy7 Conjugated

Applications

  • WB
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Cow
  • Sheep
  • Horse
  • Rabbit
Overview
Catalog # bs-17663R-Cy7
Product Name SPIB Polyclonal Antibody, Cy7 Conjugated
Applications
WB IF(ICC)
Reactivity Mouse
Predicted Reactivity Human, Rat, Cow, Sheep, Horse, Rabbit
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SPIB
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 6689
Swiss Prot Q01892
Subcellular location Cytoplasm, Nucleus
Synonyms SPI B; Spi B transcription factor; Spib; SPIB_HUMAN; Transcription factor Spi-B.
Background The protein encoded by this gene is a transcriptional activator that binds to the PU-box (5'-GAGGAA-3') and acts as a lymphoid-specific enhancer. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Application Dilution
WB 1:300-5000
IF(ICC) 1:200-400

Frequently asked questions

Which applications is SPIB Polyclonal Antibody, Cy7 Conjugated (bs-17663R-Cy7) validated for, and at what dilution?

bs-17663R-Cy7 is validated for WB (1:300-5000); IF(ICC) (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does SPIB Polyclonal Antibody, Cy7 Conjugated (bs-17663R-Cy7) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Cow, Sheep, Horse, Rabbit.

How should bs-17663R-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SPIB Polyclonal Antibody, Cy7 Conjugated (bs-17663R-Cy7)?

KLH conjugated synthetic peptide derived from human SPIB

What are the excitation and emission wavelengths of the Cy7 conjugate (bs-17663R-Cy7)?

Cy7 on bs-17663R-Cy7: excitation / emission 743nm/767nm.