SPIB Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-17663R-FITC |
| Product Name | SPIB Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Cow, Sheep, Horse, Rabbit |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SPIB |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6689 |
| Swiss Prot | Q01892 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | SPI B; Spi B transcription factor; Spib; SPIB_HUMAN; Transcription factor Spi-B. |
| Background | The protein encoded by this gene is a transcriptional activator that binds to the PU-box (5'-GAGGAA-3') and acts as a lymphoid-specific enhancer. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:200-400 |
Frequently asked questions
Which applications is SPIB Polyclonal Antibody, FITC Conjugated (bs-17663R-FITC) validated for, and at what dilution?
bs-17663R-FITC is validated for WB (1:300-5000); IF(ICC) (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SPIB Polyclonal Antibody, FITC Conjugated (bs-17663R-FITC) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Cow, Sheep, Horse, Rabbit.
How should bs-17663R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SPIB Polyclonal Antibody, FITC Conjugated (bs-17663R-FITC)?
KLH conjugated synthetic peptide derived from human SPIB
What are the excitation and emission wavelengths of the FITC conjugate (bs-17663R-FITC)?
FITC on bs-17663R-FITC: excitation / emission 494nm/518nm.