phospho-SF1 (Ser82) Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-17680R-Cy5 |
| Product Name | phospho-SF1 (Ser82) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthesised phosphopeptide derived from human splicing factor 1 around the phosphorylation site of Ser82 |
| Modification Site | Ser82 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7536 |
| Swiss Prot | Q15637 |
| Subcellular location | Nucleus |
| Synonyms | splicing factor 1 (phospho S82); p-splicing factor 1 (phospho S82); BBP; D11S636; Mammalian branch point-binding protein mBBP; OTTHUMP00000069749; OTTHUMP00000069750; OTTHUMP00000069752; splicing factor 1; Transcription factor ZFM1; ZFM1; Zinc finger gene in MEN1 locus; Zinc finger protein 162; ZNF162. |
| Background | This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence and is required for the early stages of spliceosome assembly. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010] |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-SF1 (Ser82) Polyclonal Antibody, Cy5 Conjugated (bs-17680R-Cy5) validated for, and at what dilution?
bs-17680R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-SF1 (Ser82) Polyclonal Antibody, Cy5 Conjugated (bs-17680R-Cy5) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Cow, Sheep, Pig, Horse, Rabbit.
How should bs-17680R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-SF1 (Ser82) Polyclonal Antibody, Cy5 Conjugated (bs-17680R-Cy5)?
KLH conjugated synthesised phosphopeptide derived from human splicing factor 1 around the phosphorylation site of Ser82
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-17680R-Cy5)?
Cy5 on bs-17680R-Cy5: excitation / emission 625,650nm/670nm.