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SPRED2 Polyclonal Antibody, PerCP Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Cow
  • Sheep
  • Pig
  • Rabbit
Overview
Catalog # bs-17686R-PerCP-100uL
Product Name SPRED2 Polyclonal Antibody, PerCP Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Cow, Sheep, Pig, Rabbit
Specifications
Conjugation PerCP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SPRED2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 200734
Swiss Prot Q7Z698
Subcellular location Cell membrane
Synonyms C79158; FLJ21897; FLJ31917; SPRE2_HUMAN; MGC163164; Spred 2; Sprouty protein with EVH 1 domain 2 related sequence; sprouty related EVH1 domain containing 2; Sprouty related protein with EVH 1 domain 2; zgc:77284.
Background Tyrosine kinase substrate that inhibits growth-factor-mediated activation of MAP kinase.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is SPRED2 Polyclonal Antibody, PerCP Conjugated (bs-17686R-PerCP) validated for, and at what dilution?

bs-17686R-PerCP is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does SPRED2 Polyclonal Antibody, PerCP Conjugated (bs-17686R-PerCP) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Sheep, Pig, Rabbit.

How should bs-17686R-PerCP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SPRED2 Polyclonal Antibody, PerCP Conjugated (bs-17686R-PerCP)?

KLH conjugated synthetic peptide derived from human SPRED2

What are the excitation and emission wavelengths of the PerCP conjugate (bs-17686R-PerCP)?

PerCP on bs-17686R-PerCP: excitation / emission 482nm/677nm.