Follistatin Polyclonal Antibody

$Antigen: bs-1773P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450$

Antigen: bs-1773P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450

Formalin-fixed and paraffin embedded human breast carcinoma labeled with Rabbit Anti-Follistatin Polyclonal Antibody (bs-1773R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Lane 1: HCCLM3 lysates; probed with Follistatin Polyclonal Antibody, Unconjugated (bs-1773R) at 1:300 dilution and 4˚Covernight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Mouse Pituitary tissue lysates; Lane 2: Mouse Placenta tissue lysates; Lane 3: Rat Pituitary tissue lysates; Lane 4: Rat Placenta tissue lysates ; Lane 5: Rat Breast tissue lysates probed with Follistatin Polyclonal Antibody, Unconjugated (bs-1773R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Pituitary tissue lysates; Lane 2: Mouse Placenta tissue lysates; Lane 3: Rat Pituitary tissue lysates; Lane 4: Rat Placenta tissue lysates ; Lane 5: Rat Breast tissue lysates probed with Follistatin Polyclonal Antibody, Unconjugated (bs-1773R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

ELISA
IHC-P

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Sheep
Pig
Horse
Chicken

Overview
Catalog #	bs-1773R
Product Name	Follistatin Polyclonal Antibody
Applications	ELISA, IHC-P
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Sheep, Pig, Horse, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Follistatin
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10468
Swiss Prot	P19883
Subcellular location	Secreted
Synonyms	FS; Follistatin; Activin-binding protein; FST
Background	Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH).
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400