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CCKBR Polyclonal Antibody, AbBy Fluor-350 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Cow
  • Rabbit
Overview
Catalog # bs-1777R-BF350-100uL
Product Name CCKBR Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human, Cow, Rabbit
Specifications
Conjugation AbBy Fluor-350
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Gastrin receptor
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 887
Swiss Prot P32239
Subcellular location Cell membrane
Synonyms GASR; CCK-B; CCK2R; Gastrin/cholecystokinin type B receptor; CCK-B receptor; CCK-BR; Cholecystokinin-2 receptor; CCK2-R; CCKBR; CCKRB
Background Receptor for gastrin and cholecystokinin. The CKK-B receptors occur throughout the central nervous system where they modulate anxiety, analgesia, arousal, and neuroleptic activity. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Isoform 2 is constitutively activated and may regulate cancer cell proliferation via a gastrin-independent mechanism.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is CCKBR Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-1777R-BF350) validated for, and at what dilution?

bs-1777R-BF350 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CCKBR Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-1777R-BF350) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Rabbit.

How should bs-1777R-BF350 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CCKBR Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-1777R-BF350)?

KLH conjugated synthetic peptide derived from human Gastrin receptor

What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-1777R-BF350)?

AbBy Fluor-350 on bs-1777R-BF350: excitation / emission 346nm/442nm.