GATA4 Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1778R-Cy7-100uL |
| Product Name | GATA4 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(ICC)
|
| Specificity | May also interact with GATA-5, GATA-6 |
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Pig, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human GATA-4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2626 |
| Swiss Prot | P43694 |
| Subcellular location | Nucleus |
| Synonyms | TOF; ASD2; VSD1; TACHD; Transcription factor GATA-4; GATA-binding factor 4; GATA4 |
| Background | Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development. Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression. Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions. Acts as a transcriptional activator of ANF in cooperation with NKX2-5. Promotes cardiac myocyte enlargement. Required during testicular development. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GATA4 Polyclonal Antibody, Cy7 Conjugated (bs-1778R-Cy7) validated for, and at what dilution?
bs-1778R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GATA4 Polyclonal Antibody, Cy7 Conjugated (bs-1778R-Cy7) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Chicken, Rabbit.
How should bs-1778R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GATA4 Polyclonal Antibody, Cy7 Conjugated (bs-1778R-Cy7)?
KLH conjugated synthetic peptide derived from human GATA-4
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-1778R-Cy7)?
Cy7 on bs-1778R-Cy7: excitation / emission 743nm/767nm.