MTRF1L Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-17893R-BF555 |
| Product Name | MTRF1L Polyclonal Antibody, AbBy Fluor-555 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MTRF1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 54516 |
| Swiss Prot | Q9UGC7 |
| Subcellular location | Cytoplasm |
| Synonyms | HMRF1L; Human mitochondrial release factor 1-like; Mitochondrial release factor 1 like; Mitochondrial translational release factor 1 like; Mitochondrial translational release factor 1-like; mtRF1a; MTRF1L; Peptide chain release factor 1-like, mitochondrial; RF1ML_HUMAN. |
| Background | Mitochondrial DNA encodes 13 proteins that play essential roles in the respiratory chain, while all proteins involved in mitochondrial translation are encoded by nuclear genes that are imported from the cytoplasm. MTRF1L is a nuclear-encoded protein that functions as a releasing factor that recognizes termination codons and releases mitochondrial ribosomes from the synthesized protein (summary by Nozaki et al., 2008 [PubMed 18429816]).[supplied by OMIM, Nov 2010] |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is MTRF1L Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-17893R-BF555) validated for, and at what dilution?
bs-17893R-BF555 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MTRF1L Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-17893R-BF555) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Horse, Rabbit.
How should bs-17893R-BF555 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MTRF1L Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-17893R-BF555)?
KLH conjugated synthetic peptide derived from human MTRF1
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-17893R-BF555)?
AbBy Fluor-555 on bs-17893R-BF555: excitation / emission 553nm/568nm.