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NPTN Polyclonal Antibody, FITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
Overview
Catalog # bs-1801R-FITC-100uL
Product Name NPTN Polyclonal Antibody, FITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NPTN
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 27020
Swiss Prot Q9Y639
Subcellular location Cell membrane
Synonyms stromal cell derived factor receptor 1; GP55; GP65; neuroplastin; np55; np65; SDFR1; SDR1; stromal cell derived factor receptor 1; Neuroplastin; NPTN_HUMAN; SDR-1.
Background NPTN is a type I transmembrane protein belonging to the Ig superfamily. The protein is believed to be involved in cell-cell interactions or cell-substrate interactions. The alpha and beta transcripts show differential localization within the brain.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is NPTN Polyclonal Antibody, FITC Conjugated (bs-1801R-FITC) validated for, and at what dilution?

bs-1801R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NPTN Polyclonal Antibody, FITC Conjugated (bs-1801R-FITC) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken.

How should bs-1801R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NPTN Polyclonal Antibody, FITC Conjugated (bs-1801R-FITC)?

KLH conjugated synthetic peptide derived from human NPTN

What are the excitation and emission wavelengths of the FITC conjugate (bs-1801R-FITC)?

FITC on bs-1801R-FITC: excitation / emission 494nm/518nm.