HMGN2/HMG17 Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-18054R-Biotin |
| Product Name | HMGN2/HMG17 Polyclonal Antibody, Biotin Conjugated |
| Applications |
IHC-P
IHC-F
|
| Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HMGN2/HMG17:2-60/90 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 3151 |
| Swiss Prot | P05204 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | High mobility group (nonhistone chromosomal) protein 17; high mobility group nucleosomal binding domain 2; High mobility group nucleosome-binding domain-containing protein 2; High mobility group protein N2; HMG17; HMGN2; HMGN2_HUMAN; MGC5629; Non histone chromosomal protein HMG 17; Non-histone chromosomal protein HMG-17; Nonhistone chromosomal protein HMG 17; nonhistone chromosomal protein hmg-17. |
| Background | The high-mobility group (HMG) proteins 14 and 17 are abundant chromosomal proteins that bind to nucleosomes and enhance transcription (15). HMG-14 and HMG-17 also function as architectural elements, which alter the structure of the chromatin fiber and enhance transcription from chromatin templates (13,5). HMG-14/17 proteins modify the nucleosomal organization of the 30 nm chromatin fiber and mediate the unfolding of the higher order chromatin structure thereby facilitating access to the underlying DNA sequence (13). Clustering of architectural elements, such as HMG proteins and linker histone subtypes into distinct domains, may lead to structural and functional heterogeneity along the chromatin fiber (13). In addition, HMG-14 and HMG-17 have been identified as constitutive components of mouse oocyte and embryonic chromatin that establish a link between the structure of embryonic chromatin and the normal progression of embryonic development (2). Post-translational modifications : Phosphorylation favors cytoplasmic localization. Similarity : Belongs to the HMGN family. |
| Application Dilution | |
| IHC-P | 1:200-400 |
| IHC-F | |
Frequently asked questions
Which applications is HMGN2/HMG17 Polyclonal Antibody, Biotin Conjugated (bs-18054R-Biotin) validated for, and at what dilution?
bs-18054R-Biotin is validated for IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HMGN2/HMG17 Polyclonal Antibody, Biotin Conjugated (bs-18054R-Biotin) react with?
Tested reactivity: Mouse, Rat.
How should bs-18054R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HMGN2/HMG17 Polyclonal Antibody, Biotin Conjugated (bs-18054R-Biotin)?
KLH conjugated synthetic peptide derived from human HMGN2/HMG17:2-60/90