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IL-6R alpha Polyclonal Antibody, PerCP Conjugated

Applications

  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
Overview
Catalog # bs-1805r-percp-100uL
Product Name IL-6R alpha Polyclonal Antibody, PerCP Conjugated
Applications
FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Specifications
Conjugation PerCP
Host Rabbit
Source KLH conjugated synthetic peptide derived from mouse IL-6R alpha
Immunogen Range 351-460/460
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 16194
Swiss Prot P22272
Subcellular location Secreted, Cell membrane
Synonyms Il6r; CD126; IL-6R; Interleukin-6 receptor subunit alpha; IL-6 receptor subunit alpha; IL-6R subunit alpha; IL-6R-alpha; IL-6RA; IL-6R 1; Il6ra
Background Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.
Application Dilution
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is IL-6R alpha Polyclonal Antibody, PerCP Conjugated (bs-1805R-PerCP) validated for, and at what dilution?

bs-1805R-PerCP is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does IL-6R alpha Polyclonal Antibody, PerCP Conjugated (bs-1805R-PerCP) react with?

Tested reactivity: Human.

How should bs-1805R-PerCP be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise IL-6R alpha Polyclonal Antibody, PerCP Conjugated (bs-1805R-PerCP)?

KLH conjugated synthetic peptide derived from mouse IL-6R alpha Immunogen range: 351-460/460.

What are the excitation and emission wavelengths of the PerCP conjugate (bs-1805R-PerCP)?

PerCP on bs-1805R-PerCP: excitation / emission 482nm/677nm.