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HSPA12A Polyclonal Antibody, Cy5.5 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-18076R-Cy5.5
Product Name HSPA12A Polyclonal Antibody, Cy5.5 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human HSPA12A
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 259217
Swiss Prot O43301
Subcellular location Cytoplasm
Synonyms FLJ13874; Heat shock 70 kDa protein 12A; heat shock 70kD protein 12A; heat shock 70kDa protein 12A; HS12A_HUMAN; Hspa12a; KIAA0417
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is HSPA12A Polyclonal Antibody, Cy5.5 Conjugated (BS-18076R-CY5-5) validated for, and at what dilution?

BS-18076R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does HSPA12A Polyclonal Antibody, Cy5.5 Conjugated (BS-18076R-CY5-5) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit.

How should BS-18076R-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise HSPA12A Polyclonal Antibody, Cy5.5 Conjugated (BS-18076R-CY5-5)?

KLH conjugated synthetic peptide derived from human HSPA12A

What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-18076R-CY5-5)?

Cy5.5 on BS-18076R-CY5-5: excitation / emission 675nm/694nm.