phospho-IKB alpha (Ser36) Polyclonal Antibody, APC-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-18129R-APC-Cy5.5-100uL |
| Product Name | phospho-IKB alpha (Ser36) Polyclonal Antibody, APC-Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Cow, Sheep, Pig, Rabbit |
| Specifications | |
| Conjugation | APC-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human phospho-IKB alpha (Ser36) |
| Modification Site | Ser36 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4792 |
| Swiss Prot | P25963 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | IKB alpha (phospho S36); p-IKB alpha (phospho S36); I kappa B alpha; I-kappa-B-alpha; IkappaBalpha; IkB-alpha; IKBA; IKBA_HUMAN; IKBalpha; MAD 3; MAD3; Major histocompatibility complex enhancer-binding protein MAD3; NF kappa B inhibitor alpha; NF-kappa-B inhibitor alpha; NFKBI; NFKBIA; Nuclear factor of kappa light chain gene enhancer in B cells; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha. |
| Background | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-IKB alpha (Ser36) Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-18129R-APC-CY5-5) validated for, and at what dilution?
BS-18129R-APC-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-IKB alpha (Ser36) Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-18129R-APC-CY5-5) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Cow, Sheep, Pig, Rabbit.
How should BS-18129R-APC-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-IKB alpha (Ser36) Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-18129R-APC-CY5-5)?
KLH conjugated synthetic peptide derived from human phospho-IKB alpha (Ser36)
What are the excitation and emission wavelengths of the APC-Cy5.5 conjugate (BS-18129R-APC-CY5-5)?
APC-Cy5.5 on BS-18129R-APC-CY5-5: excitation / emission 650nm/694nm.