HRG1 Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1814R-Cy7-100uL |
| Product Name | HRG1 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat, Zebrafish |
| Predicted Reactivity | Dog, Pig, Horse, Chicken, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Pro-Neuregulin 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3084 |
| Swiss Prot | Q02297 |
| Subcellular location | Nucleus, Secreted, Cell membrane |
| Synonyms | GGF; HGL; HRG; NDF; ARIA; GGF2; HRG1; HRGA; SMDF; MST131; MSTP131; NRG1-IT2; Pro-neuregulin-1, membrane-bound isoform; Pro-NRG1; NRG1 |
| Background | Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is HRG1 Polyclonal Antibody, Cy7 Conjugated (bs-1814R-Cy7) validated for, and at what dilution?
bs-1814R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HRG1 Polyclonal Antibody, Cy7 Conjugated (bs-1814R-Cy7) react with?
Tested reactivity: Human, Mouse, Rat, Zebrafish. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Pig, Horse, Chicken, Rabbit, GuineaPig.
How should bs-1814R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HRG1 Polyclonal Antibody, Cy7 Conjugated (bs-1814R-Cy7)?
KLH conjugated synthetic peptide derived from human Pro-Neuregulin 1
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-1814R-Cy7)?
Cy7 on bs-1814R-Cy7: excitation / emission 743nm/767nm.