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IL34 Polyclonal Antibody, APC-Cy7 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-18170R-APC-Cy7
Product Name IL34 Polyclonal Antibody, APC-Cy7 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation APC-Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human IL34
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 76527
Swiss Prot Q8R1R4
Subcellular location Secreted
Synonyms AI593503; 2010004A03Rik; Interleukin-34; IL-34; Il34
Background Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1 (By similarity).
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is IL34 Polyclonal Antibody, APC-Cy7 Conjugated (bs-18170R-APC-Cy7) validated for, and at what dilution?

bs-18170R-APC-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does IL34 Polyclonal Antibody, APC-Cy7 Conjugated (bs-18170R-APC-Cy7) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit.

How should bs-18170R-APC-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise IL34 Polyclonal Antibody, APC-Cy7 Conjugated (bs-18170R-APC-Cy7)?

KLH conjugated synthetic peptide derived from human IL34

What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-18170R-APC-Cy7)?

APC-Cy7 on bs-18170R-APC-Cy7: excitation / emission 650nm/780nm.