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ISX/Intestine specific homeobox Polyclonal Antibody, RBITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Cow
  • Sheep
  • Horse
Overview
Catalog # bs-18172R-RBITC
Product Name ISX/Intestine specific homeobox Polyclonal Antibody, RBITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse
Predicted Reactivity Rat, Cow, Sheep, Horse
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ISX/Intestine specific homeobox
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 91464
Swiss Prot Q2M1V0
Subcellular location Nucleus
Synonyms DKFZp781N2395; Intestine-specific homeobox; Isx; ISX_HUMAN; MGC138417; OTTHUMP00000028558; Pancreas intestine homeodomain transcription factor; Pix 1; RAX like homeobox; RAX-like homeobox; RAXLX.
Background Homeobox genes encode DNA-binding proteins, many of which are thought to be involved in early embryonic development. Homeobox genes encode a DNA-binding domain of 60 to 63 amino acids referred to as the homeodomain. This gene is a member of the RAXLX homeobox gene family. [provided by RefSeq, Jul 2008]
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is ISX/Intestine specific homeobox Polyclonal Antibody, RBITC Conjugated (bs-18172R-RBITC) validated for, and at what dilution?

bs-18172R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ISX/Intestine specific homeobox Polyclonal Antibody, RBITC Conjugated (bs-18172R-RBITC) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Cow, Sheep, Horse.

How should bs-18172R-RBITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ISX/Intestine specific homeobox Polyclonal Antibody, RBITC Conjugated (bs-18172R-RBITC)?

KLH conjugated synthetic peptide derived from human ISX/Intestine specific homeobox

What are the excitation and emission wavelengths of the RBITC conjugate (bs-18172R-RBITC)?

RBITC on bs-18172R-RBITC: excitation / emission 540nm/625nm.