Calcitonin Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-18210R-Cy5.5 |
| Product Name | Calcitonin Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Calcitonin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 796 |
| Swiss Prot | P01258 |
| Subcellular location | Secreted |
| Synonyms | Alpha CGRP; CALC 1; CALC A; CALC1; CALCA; Calcitonin related polypeptide alpha; CGRP 1; CGRP; CGRP I; CGRP-1; CGRPI; CGRP1; CT; Katacalcin; KC; MGC126648; alpha type CGRP; calcitonin 1; calcitonin gene related peptide 1; calcitonin gene related peptide I; calcitonin isoform CT preproprotein. |
| Background | Calcitonin is a 32 amino acid peptide hormone synthesized by the parafollicular cells of the thyroid. It causes a rapid, but short lived, reduction in serum calcium and phosphate by promoting the incorporation of those ions in the bones. This effect is opposite to that of parathyroid hormone. Staining for calcitonin may be used for the identification of a spectrum of C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors. Staining for calcitonin in medullary carcinoma of the thyroid produces a fine granular pattern in the cytoplasm. Amyloid deposits within the tumor may also exhibit varying degrees of calcitonin activity. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Calcitonin Polyclonal Antibody, Cy5.5 Conjugated (BS-18210R-CY5-5) validated for, and at what dilution?
BS-18210R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Calcitonin Polyclonal Antibody, Cy5.5 Conjugated (BS-18210R-CY5-5) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Rabbit, GuineaPig.
How should BS-18210R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Calcitonin Polyclonal Antibody, Cy5.5 Conjugated (BS-18210R-CY5-5)?
KLH conjugated synthetic peptide derived from human Calcitonin
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-18210R-CY5-5)?
Cy5.5 on BS-18210R-CY5-5: excitation / emission 675nm/694nm.