ICAM3 Polyclonal Antibody
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-1821R |
| Product Name | ICAM3 Polyclonal Antibody |
| Applications |
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CD50 |
| Immunogen Range | 30-130/547 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 3385 |
| Subcellular location | Cell membrane |
| Synonyms | intercellular adhesion molecules-3; ICAM-3; ICAM 3; CD50; CDW 50; CDW50; ICAM 3; ICAM R; ICAMR. |
| Background | All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA1 protein. ICAM3 is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. Upon stimulation by a physiological stimulus it becomes rapidly and transiently phosphorylated on serine residues. N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ICAM3 Polyclonal Antibody (bs-1821R) validated for, and at what dilution?
bs-1821R is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200); ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-1821R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ICAM3 Polyclonal Antibody (bs-1821R)?
KLH conjugated synthetic peptide derived from human CD50 Immunogen range: 30-130/547.