LGI2 Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-18235R-Biotin-100uL |
| Product Name | LGI2 Polyclonal Antibody, Biotin Conjugated |
| Applications |
WB
ELISA
IHC-P
IHC-F
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Cow, Sheep, Pig, Horse, Chicken, GuineaPig |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human LGI2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 55203 |
| Swiss Prot | Q8N0V4 |
| Subcellular location | Secreted |
| Synonyms | KIAA1916; Leucine rich glioma inactivated protein 2; Leucine rich repeat LGI family member 2; Leucine-rich glioma-inactivated protein 2; Leucine-rich repeat LGI family member 2; LGI1 like protein 2; LGI1-like protein 2; LGI2; LGI2_HUMAN; LGIL2. |
| Background | The leucine-rich (LRR) repeat is a 20-30 amino acid motif that forms a hydrophobic Alpha/Beta horseshoe fold, allowing it to accommodate several leucine residues within a tightly packed core. All LRR repeats contain a variable segment and a highly conserved segment, the latter of which accounts for 11 or 12 residues of the entire LRR motif. LGI2 (leucine-rich repeat LGI family, member 2), also known as KIAA1916 or LGIL2, is a 545 amino acid secreted protein that contains four LRR repeats and seven EAR repeats. Expressed in heart, brain and placenta, LGI2 shares high sequence similarity with other LGI family members and is thought to play a role in the pathogenesis of epileptic disorders. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is LGI2 Polyclonal Antibody, Biotin Conjugated (bs-18235R-Biotin) validated for, and at what dilution?
bs-18235R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does LGI2 Polyclonal Antibody, Biotin Conjugated (bs-18235R-Biotin) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Cow, Sheep, Pig, Horse, Chicken, GuineaPig.
How should bs-18235R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise LGI2 Polyclonal Antibody, Biotin Conjugated (bs-18235R-Biotin)?
KLH conjugated synthetic peptide derived from human LGI2