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LIAS Polyclonal Antibody, AbBy Fluor-350 Conjugated

Applications

  • WB
  • IF

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-18247r-bf350-100uL
Product Name LIAS Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
WB IF
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation AbBy Fluor-350
Host Rabbit
Source KLH conjugated synthetic peptide derived from human LIAS
Immunogen Range 28-130/372
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 11019
Swiss Prot O43766
Synonyms HUSSY 01; LAS; LIP 1; Lip syn; LIP1; Lipoate synthase; Lipoic acid synthetase; Lipoic acid synthetase mitochondrial; Lipoyl synthase, mitochondrial; LS; MGC23245.
Background The protein encoded by this gene belongs to the biotin and lipoic acid synthetases family. It localizes in mitochondrion and plays an important role in alpha-(+)-lipoic acid synthesis. It may also function in the sulfur insertion chemistry in lipoate biosynthesis. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]
Application Dilution
WB 1:500-2000
IF 1:100-500

Frequently asked questions

Which applications is LIAS Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-18247R-BF350) validated for, and at what dilution?

bs-18247R-BF350 is validated for WB (1:500-2000); IF (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does LIAS Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-18247R-BF350) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit.

How should bs-18247R-BF350 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise LIAS Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-18247R-BF350)?

KLH conjugated synthetic peptide derived from human LIAS Immunogen range: 28-130/372.

What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-18247R-BF350)?

AbBy Fluor-350 on bs-18247R-BF350: excitation / emission 346nm/442nm.