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LIAS Polyclonal Antibody, Biotin Conjugated

Applications

  • WB
  • IHC-P
  • IHC-F

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-18247r-biotin-100uL
Product Name LIAS Polyclonal Antibody, Biotin Conjugated
Applications
WB IHC-P IHC-F
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic peptide derived from human LIAS
Immunogen Range 28-130/372
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 11019
Swiss Prot O43766
Synonyms HUSSY 01; LAS; LIP 1; Lip syn; LIP1; Lipoate synthase; Lipoic acid synthetase; Lipoic acid synthetase mitochondrial; Lipoyl synthase, mitochondrial; LS; MGC23245.
Background The protein encoded by this gene belongs to the biotin and lipoic acid synthetases family. It localizes in mitochondrion and plays an important role in alpha-(+)-lipoic acid synthesis. It may also function in the sulfur insertion chemistry in lipoate biosynthesis. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]
Application Dilution
WB 1:500-2000
IHC-P 1:100-500
IHC-F 1:100-500

Frequently asked questions

Which applications is LIAS Polyclonal Antibody, Biotin Conjugated (bs-18247R-Biotin) validated for, and at what dilution?

bs-18247R-Biotin is validated for WB (1:500-2000); IHC-P (1:100-500); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does LIAS Polyclonal Antibody, Biotin Conjugated (bs-18247R-Biotin) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit.

How should bs-18247R-Biotin be stored?

Store at -20°C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise LIAS Polyclonal Antibody, Biotin Conjugated (bs-18247R-Biotin)?

KLH conjugated synthetic peptide derived from human LIAS Immunogen range: 28-130/372.