LIM2 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-18272R-Cy5.5 |
| Product Name | LIM2 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human LIM2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3982 |
| Swiss Prot | P55344 |
| Subcellular location | Cell membrane |
| Synonyms | Lens fiber membrane intrinsic protein; Lens intrinsic membrane protein 2 19kDa; Lens intrinsic membrane protein 2; LIM 2; Lim2; LMIP_HUMAN; MP17; MP18; MP19; MP20; To3. |
| Background | This gene encodes an eye lens-specific protein found at the junctions of lens fiber cells, where it may contribute to cell junctional organization. It acts as a receptor for calmodulin, and may play an important role in both lens development and cataractogenesis. Mutations in this gene have been associated with cataract formation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009] |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is LIM2 Polyclonal Antibody, Cy5.5 Conjugated (BS-18272R-CY5-5) validated for, and at what dilution?
BS-18272R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does LIM2 Polyclonal Antibody, Cy5.5 Conjugated (BS-18272R-CY5-5) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Horse, Rabbit.
How should BS-18272R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise LIM2 Polyclonal Antibody, Cy5.5 Conjugated (BS-18272R-CY5-5)?
KLH conjugated synthetic peptide derived from human LIM2
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-18272R-CY5-5)?
Cy5.5 on BS-18272R-CY5-5: excitation / emission 675nm/694nm.