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Liprin alpha 2 Polyclonal Antibody, RBITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-18299R-RBITC
Product Name Liprin alpha 2 Polyclonal Antibody, RBITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Liprin alpha 2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 8499
Swiss Prot O75334
Subcellular location Cell membrane
Synonyms LIPA2_HUMAN; Liprin-alpha-2; PPFIA2; Protein tyrosine phosphatase receptor type f polypeptide interacting protein alpha 2; Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-2; Protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2; PTPRF interacting protein alpha 2; PTPRF-interacting protein alpha-2.
Background The protein encoded by this gene is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. It has been proposed that liprins are multivalent proteins that form complex structures and act as scaffolds for the recruitment and anchoring of LAR family of tyrosine phosphatases. This protein has been shown to bind the calcium/calmodulin-dependent serine protein kinase (MAGUK family) protein (also known as CASK) and proposed to regulate higher-order brain functions in mammals. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Liprin alpha 2 Polyclonal Antibody, RBITC Conjugated (bs-18299R-RBITC) validated for, and at what dilution?

bs-18299R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Liprin alpha 2 Polyclonal Antibody, RBITC Conjugated (bs-18299R-RBITC) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Pig, Horse, Chicken, Rabbit.

How should bs-18299R-RBITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Liprin alpha 2 Polyclonal Antibody, RBITC Conjugated (bs-18299R-RBITC)?

KLH conjugated synthetic peptide derived from human Liprin alpha 2

What are the excitation and emission wavelengths of the RBITC conjugate (bs-18299R-RBITC)?

RBITC on bs-18299R-RBITC: excitation / emission 540nm/625nm.