SAKS1/2B28 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SAKS1\/2B28 Polyclonal Antibody, Unconjugated (bs-18331R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SAKS1\/2B28 Polyclonal Antibody, Unconjugated (bs-18331R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Predicted Reactivity

Human
Mouse
Cow
Sheep
Pig
Horse

Overview
Catalog #	bs-18331R
Product Name	SAKS1/2B28 Polyclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Rat
Predicted Reactivity	Human, Mouse, Cow, Sheep, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human SAKS1/2B28
Immunogen Range	51-150/297
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	51035
Swiss Prot	Q04323
Subcellular location	Cytoplasm
Synonyms	LOC51035 2B28; 4930455J02Rik; D19Ertd721e; MGC124706; MGC6696; mY33K; UBXN1_HUMAN; Protein 2B28; RGD1309471; SAKS1; SAPK substrate protein 1; T25529; UBA/UBX 33.3 kDa protein; UBX domain containing protein 1; UBX domain protein 1; UBXD10; UBXN1.
Background	2B28 is a component of a complex composed of NGLY1, SAKS1, AMFR, VCP and RAD23B, where it acts as an adapter that directs VCP to polyubiquitinated proteins. 2B28 also interacts with Homer 2, which further suggests role for 2B28 in the regulation of protein degradation by ubiquitin-proteasome systems.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500

Applications

Reactivity

Predicted Reactivity

Search our catalog of hundreds of thousands of products