Help

SAKS1/2B28 Polyclonal Antibody, AbBy Fluor-350 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
  • Cow
  • Sheep
  • Pig
  • Horse
Overview
Catalog # bs-18331R-BF350
Product Name SAKS1/2B28 Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Rat
Predicted Reactivity Human, Mouse, Cow, Sheep, Pig, Horse
Specifications
Conjugation AbBy Fluor-350
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SAKS1/2B28
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 51035
Swiss Prot Q04323
Subcellular location Cytoplasm
Synonyms LOC51035 2B28; 4930455J02Rik; D19Ertd721e; MGC124706; MGC6696; mY33K; UBXN1_HUMAN; Protein 2B28; RGD1309471; SAKS1; SAPK substrate protein 1; T25529; UBA/UBX 33.3 kDa protein; UBX domain containing protein 1; UBX domain protein 1; UBXD10; UBXN1.
Background 2B28 is a component of a complex composed of NGLY1, SAKS1, AMFR, VCP and RAD23B, where it acts as an adapter that directs VCP to polyubiquitinated proteins. 2B28 also interacts with Homer 2, which further suggests role for 2B28 in the regulation of protein degradation by ubiquitin-proteasome systems.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is SAKS1/2B28 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-18331R-BF350) validated for, and at what dilution?

bs-18331R-BF350 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does SAKS1/2B28 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-18331R-BF350) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Cow, Sheep, Pig, Horse.

How should bs-18331R-BF350 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SAKS1/2B28 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-18331R-BF350)?

KLH conjugated synthetic peptide derived from human SAKS1/2B28

What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-18331R-BF350)?

AbBy Fluor-350 on bs-18331R-BF350: excitation / emission 346nm/442nm.