LXR alpha + LXR beta Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-18451R-Cy7 |
| Product Name | LXR alpha + LXR beta Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit, Xenopuslaevis |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human LXR alpha + LXR beta |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10062 |
| Swiss Prot | P55055 |
| Subcellular location | Nucleus |
| Synonyms | Liver X receptor alpha; Liver X receptor alpha; Liver X receptor beta; LXR a; LXR; LXR b; LXRa; LXRalpha; LXRb; LXRbeta; NER; NERI; NR1H2; NR1H3; Nuclear orphan receptor LXR alpha; Nuclear orphan receptor LXR beta; Nuclear receptor NER; Nuclear receptor subfamily 1 group H member 2; Nuclear receptor subfamily 1 group H member 3; Oxysterols receptor LXR alpha; Oxysterols receptor LXR beta; RIP15; RLD1; Ubiquitously expressed nuclear receptor; UNR. |
| Background | LXR alpha and LXR beta are orphan receptors. Interaction with RXR shifts RXR from its role as a silent DNA binding partner to an active ligand binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4 type response elements characterized by direct repeats of two similar hexanuclotide half sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis. LXR alpha: Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals. LXR beta: Ubiquitously expressed. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is LXR alpha + LXR beta Polyclonal Antibody, Cy7 Conjugated (bs-18451R-Cy7) validated for, and at what dilution?
bs-18451R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does LXR alpha + LXR beta Polyclonal Antibody, Cy7 Conjugated (bs-18451R-Cy7) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit, Xenopuslaevis.
How should bs-18451R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise LXR alpha + LXR beta Polyclonal Antibody, Cy7 Conjugated (bs-18451R-Cy7)?
KLH conjugated synthetic peptide derived from human LXR alpha + LXR beta
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-18451R-Cy7)?
Cy7 on bs-18451R-Cy7: excitation / emission 743nm/767nm.