NR3C2/Mineralocorticoid receptor Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1850R-RBITC |
| Product Name | NR3C2/Mineralocorticoid receptor Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Pig, Chicken, Rabbit |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Mineralocorticoid receptor |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4306 |
| Swiss Prot | P08235 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | MR; MCR; MLR; NR3C2VIT; Mineralocorticoid receptor; Nuclear receptor subfamily 3 group C member 2; NR3C2 |
| Background | Receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. Binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is NR3C2/Mineralocorticoid receptor Polyclonal Antibody, RBITC Conjugated (bs-1850R-RBITC) validated for, and at what dilution?
bs-1850R-RBITC is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NR3C2/Mineralocorticoid receptor Polyclonal Antibody, RBITC Conjugated (bs-1850R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Chicken, Rabbit.
How should bs-1850R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NR3C2/Mineralocorticoid receptor Polyclonal Antibody, RBITC Conjugated (bs-1850R-RBITC)?
KLH conjugated synthetic peptide derived from human Mineralocorticoid receptor
What are the excitation and emission wavelengths of the RBITC conjugate (bs-1850R-RBITC)?
RBITC on bs-1850R-RBITC: excitation / emission 540nm/625nm.