DUSP4 Polyclonal Antibody, PerCP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-1852R-PerCP-100uL |
| Product Name | DUSP4 Polyclonal Antibody, PerCP Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Chicken, Rabbit |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MKP-2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1846 |
| Subcellular location | Nucleus |
| Synonyms | Mitogen activated protein kinase phosphatase 2; Dual specicity phosphatase 4; Dual specicity protein phosphatase 4; Dual specicity protein phosphatase hVH2; DUSP 4; HVH 2; HVH2; MAP kinase phosphatase 2; Mitogen activated protein kinase phosphatase 2; MKP 2; MKP2; Serine/threonine specic protein phosphatase; TYP; VH 2; VH1 homologous phosphatase 2; VH2. |
| Background | DUSP4 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. Two alternatively spliced transcript variants, encoding distinct isoforms, have been observed for this gene. In addition, multiple polyadenylation sites have been reported. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DUSP4 Polyclonal Antibody, PerCP Conjugated (bs-1852R-PerCP) validated for, and at what dilution?
bs-1852R-PerCP is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DUSP4 Polyclonal Antibody, PerCP Conjugated (bs-1852R-PerCP) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Chicken, Rabbit.
How should bs-1852R-PerCP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DUSP4 Polyclonal Antibody, PerCP Conjugated (bs-1852R-PerCP)?
KLH conjugated synthetic peptide derived from human MKP-2
What are the excitation and emission wavelengths of the PerCP conjugate (bs-1852R-PerCP)?
PerCP on bs-1852R-PerCP: excitation / emission 482nm/677nm.